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疫苗工艺研究

1 A predictive high-throughput scale-down model of monoclonal antibody production in CHO cells.pdf
2 A serum-free Vero production platform for a chimeric virus vaccine candidate.pdf
3 Developing an Industrial Artemisinic Acid Fermentation Process to support the cost-effective production of antimalarial artemisinin based combination therapies.pdf
4 Enhanced cell culture performance using inducible anti‐apoptotic genes E1B‐19K and Aven in the production of a monoclonal antibody with Chinese hamster ovary.pdf
5 Enhanced kinetic extraction of parvovirus B19 structural proteins.pdf
6 Expression and purification of an influenza hemagglutinin—one step closer to a recombinant protein-based influenza vaccine.pdf
7 Fed-batch cultivation of Bordetella pertussis Metabolism and Pertussis Toxin production.pdf
8 Improving the cellular pertussis vaccine increased potency and consistency.pdf
9 Modeling Neisseria meningitidis metabolism from genome to metabolic fluxes.pdf
10 Novel micro‐bioreactor high throughput technology for cell culture process development Reproducibility and scalability assessment of fed‐batch CHO cultures.pdf

干细胞培养 

1 Application_UNIV_TORONTO_Perfusion_Paper_BIOTECHNOLOGY_AND_BIOENGENEERING_2005.pdf

2 Online- and offline- monitoring of stem cell expansion on microcarrier.pdf

3 Patterns of amino acid metabolism by proliferating human mesenchymal stem cells.pdf

4 SLI 6pp Bioproducts 2013.pdf

微载体培养
1 Bioprocess Equipment Characterization of Energy Dissipation Rate and Its Potential to Damage Cells.pdf
2 Bioreactor design and bioprocess controls for industrialized cell processing.pdf
3 Bubble-column design for growth of fragile insect cells.pdf
4 CＨＯ细胞无血清结团灌流培养：.pdf
5  Culturing Primary Brain Astrocytes Under a Fully Controlled Environment in a Novel Bioreactor.pdf
6  Gene Transfection of HEK Cells on Supermacroporous Polyacrylamide Monoliths A comparison of transient and stable recombinant protein expression in perfusion culture..pdf
7  Lipoprotein Receptor Binding, Cellular Uptake, and Lysosomal Delivery of Fusions between the Receptor-associated Protein (RAP) and α-L-Iduronidase or Acid α-Glucosidase.pdf
8  Sparged Animal Cell Bioreactors Mechanism of Cell Damage and Pluronic F-68 Protection.pdf
9  Study of the mixing time evolution during the scale.pdf
10  生物反应器培养cho细胞的生长与代谢研究.pdf
11 应用生物反应器培养Vero细胞制备甲肝病毒.pdf

CHO培养

1 A predictive high-throughput scale-down model of monoclonal antibody production in CHO cells.pdf
2  CHO expression of a novel human recombinant IgG1 anti-RhD antibody isolated by phage display.pdf
3  Dependence on glucose limitation of the pCO2 influences on CHO cell growth, metabolism and IgG production.pdf
4  Enhanced cell culture performance using inducible anti-apoptotic genesE1B-19K and Aven in the production of a monoclonal antibody with Chinese hamster ovary cells.pdf
5  Long-term stable production of monocyte-colony from CHO microcarrier perfusion cultures inhibition factor (M-CIF).pdf
6 Novel micro-bioreactor high throughput technology for cell culture process development Reproducibility and scalability assessment of fed-batch CHO cultures.pdf
7  On-line heat flux measurements improve the culture medium for the growth and productivity of genetically engineered CHO cells.pdf
8  Optimization of fed-batch parameters and harvest time of CHO cell cultures for a glycosylated product with multiple mechanisms of inactivation.pdf

昆虫细胞培养

1 High cell density fed batch and perfusion processes for stable non-viral expression of secreted alkaline phosphatase (SEAP) using insect cells comparison to a batch Sf9 BEV system..pdf

2 Optimization of a feed medium for fed-batch culture of insect cells using a genetic algorithm.pdf

3 Production of a self-activating CBM-factor X fusion protein in a stable transformed Sf9 insect cell line using high cell density perfusion culture..pdf

4 The titerless infected-cells preservation and scale-up (TIPS) method for large-scale production of NO-sensitive human soluble guanylate cyclase (sGC) from insect cells infected with recombinant baculovirus.pdf

E.coli 培养

1 A high cell density fermentation strategy to produce recombinant malarial antigen in E. coli.pdf
2 Bioproduction and characterization of a pH responsive self-assembling peptide.pdf
3 Bioproduction of lauryl lactone and 4-vinyl guaiacol as value-added chemicals.pdf
4 Combined transcriptome and proteome analysis of Escherichia coli during high cell density culture.pdf
5 DNA plasmid production in different host strains of Escherichia coli.pdf
6 E. coli K5 fermentation and the preparation of heparosan, a bioengineered heparin precursor.pdf
7 Evaluation of different expression systems for the heterologous expression of pyranose 2-oxidase from tranetes muticolor in e coli.pdf
8 Framework for online optimization of recombinant protein expression in high-cell-density Escherichia coli cultures using GFP-fusion monitoring.pdf
9 Generating Controlled Reducing Environments in Aerobic Recombinant Escherichia coli Fermentations Effects On Cell Growth, Oxygen Uptake, Heat Shock Protein Expression, and In VivoCAT Activity.pdf
10 High level production of the Magnaporthe grisea fructose 1,6-bisphosphate aldolase enzyme in Escherichia coli using a small volume bench-top fermentor.pdf
11 High-Level Production of Amorpha-4,11-Diene, a percursor of the antimalarial agent artemisinin, in Ecoli.pdf
12 Maximization of recombinant Helicobacter pylori neutrophil activating protein production in Escherichia coli improvement of a chemically defined medium using response surface methodology.pdf

BioSep声频灌流

1 A comparison of intensive cell culture bioreactors operating with Hybridomas modified for inhibited apoptotic response.pdf
2 Acoustic cell filter a proven cell retention technology for perfusion of animal cell cultures.pdf
3 Bioprocess optimization using design‐of‐experiments methodology.pdf
4 Dependence on glucose limitation of the pCO2 influences on CHO cell growth, metabolism and IgG production.pdf
5 Effect of feed and bleed rate on hybridoma cells in an acoustic perfusion bioreactor Part I. Cell density, viability, and cell‐cycle distribution.pdf
6 Integrating Acoustic Perfusion in Mammalian Cell Culture Temperature Control.pdf
7 Kinetics and manipulation of hyphal breakage and its effect on antibiotic production.pdf
8 Measurement and Control of Viable Cell Density in a Mammalian Cell Bioprocessing Facility Validation of the Software.pdf
9 Optimization and control of perfusion cultures using a viable cell probe and cell specific perfusion rates.pdf
10 Production of a self-activating CBM-factor X fusion protein in a stable transformed Sf9 insect cell line using high cell density perfusion culture.pdf
11 Retroviral vector production using suspension‐adapted 293GPG cells in a 3L acoustic filter‐based perfusion bioreactor.pdf
12 Scale-up and optimization of an acoustic filter for 200L per day perfusion of a CHO.pdf

蛋白表达
1  Effect of culture conditions on glycosylation of recombinant beta-interferon in CHO cells.pdf
2  High yields of monomeric recombinant β‐interferon from macroporous microcarrier cultures under hypothermic conditions.pdf
3 Long-term stable production of monocyte-colony inhibition factor (M-CIF) from CHO microcarrier perfusion cultures.pdf
4 Production and Glycosylation of Recombinant β-Interferon in Suspension and Cytopore Microcarrier Cultures of CHO Cells.pdf
5  Role of environmental conditions on the expression levels, glycoform pattern and levels of sialyltransferase for hFSH produced by recombinant CHO cells.pdf